In contrast with conventional CRISPR approaches, CADI does not generate double-stranded breaks or rely on inefficient homologous recombination repair mechanisms

1: CADI system is transfected into cells with transposases inactivated due to binding with nuclease

2: Nucleases bind adjacent to target region, change conformation and release transposases to form an active dimer

3: Transposase efficiently inserts large DNA fragment at target region

4: Editing complex is displaced and returns to inactive state leaving completed edit with no DNA breaks or scars